AN ABSTRACT OF THE THESIS OF COLLEEN ANN MCLEAN-BOWEN for the degree of DOCTOR OF PHILOSOPHY
نویسنده
چکیده
The role of sterols as structural components in the mitochondria] membrane of Saccharomyces cerevisiae was investigated. Gel to liquidcrystalline transitions of mitochondria] membrane preparations from mutant and wild type cells were probed using the technique of fluorescence polarization. Mitochondria isolated from those strains accumulating sterols other than ergosterol exhibited a phase transition; wild types did not. The technique was extended to an artificial membrane system using a series of sterols with modifications in the hydroxyl moiety, the ring system, and the side chain to investigate the contribution of various sterol structures to membrane fluidity. Sterols possessing a planar ring, a R-hydroxyl and a saturated side chain conferred maximal order to the bilayer. Arrhenius kinetics of the mitochondrial membrane-bound enzyme L-kynurenine-3-hydroxylase paralleled changes in fluidity observed in the fluorescence study. Examination of sphaeroplast and mitochondrial response to osmotic strength showed no correlation between sterol structure and ability to resist stretch-
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